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human granzyme b duoset elisa kit  (R&D Systems)


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    R&D Systems human granzyme b duoset elisa kit
    Human Granzyme B Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/granzyme+b/Human+Granzyme+B+DuoSet+ELISA/us12611429-3340-18-24
    Average 96 stars, based on 86 article reviews
    human granzyme b duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Validation of neoepitope presentation
    Article Snippet: Based on the intensity of color formation, the amount of Granzyme B can be quantified (see, https://www.rndsystems.com/products/human-granzyme-b-elispot-kit_el2906#product-details; Malyguine et al., Cells (2012) 1(2): 111-126).

    Article Title: Axicabtagene ciloleucel in combination with rituximab for refractory large B cell lymphoma: the phase 2, single-arm ZUMA-14 trial.
    Article Snippet: Additional serum analytes were measured using the ProteinSimple (a Bio-Techne brand) human granzyme B Simple Plex Ella assay for granzyme B, the R&D Systems (a Bio-Techne brand) Quantikine immunoassays for IL-2Rα, IL-1RA and vascular endothelial growth factor and the Roche Immunoanalyzer ferritin assay for human ferritin.

    Article Title: Axicabtagene ciloleucel in combination with rituximab for refractory large B cell lymphoma: the phase 2, single-arm ZUMA-14 trial
    Article Snippet: Additional serum analytes were measured using the ProteinSimple (a Bio-Techne brand) human granzyme B Simple Plex Ella assay for granzyme B, the R&D Systems (a Bio-Techne brand) Quantikine immunoassays for IL-2Rα, IL-1RA and vascular endothelial growth factor and the Roche Immunoanalyzer ferritin assay for human ferritin.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Processes for production of tumor infiltrating lymphocytes and uses of same in immunotherapy
    Article Snippet: .. Media supernatants restimulated as described in 5.2.5.3 were also evaluated for their levels of Granzyme B using the Human Granzyme B DuoSet ELISA Kit (R & D Systems, Minneapolis, MN) according to the manufacturer's instructions. ..

    Article Title: REACtiVe-2: phase I evaluation of dendritic cell vaccination and agonistic CD40 therapy following (m)FOLFIRINOX in metastatic pancreatic cancer
    Article Snippet: .. IFN-γ (#DY285B-05, R&D Systems) and Granzyme B (#DY2906-05, R&D Systems) levels in the supernatant were detected using ELISA. ..

    Article Title: Processes for production of tumor infiltrating lymphocytes and uses of same in immunotherapy
    Article Snippet: .. CD28, and CD137/4-1BB were also evaluated for their levels of Granzyme B using the Human Granzyme B DuoSet ELISA Kit (R & D Systems, Minneapolis, MN) according to the manufacturer's instructions. ..

    Article Title: Targeting immune cells in the aged brain reveals that engineered cytokine IL-10 enhances neurogenesis and improves cognition.
    Article Snippet: .. IFN-γ (ELISA MAXTM Deluxe Set Mouse, Biolegend, 439404), IFN-α (ELISA MAXTM Deluxe Set Mouse IFN-α1, Biolegend, 447904), IFN-β (ELISA MAXTM Deluxe Set Mouse IFN-β, Biolegend, 439404) and granzyme B (Mouse Granzyme B DuoSet ELISA, R&D Systems, DY1865-05) proteins were measured by ELISA as per the manufacturer’s instructions. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Pembrolizumab and stereotactic body radiotherapy combined in advanced hepatocellular carcinoma post sorafenib – A phase II trial (PEMRAD)
    Article Snippet: For both PD-L1 and TIL, scoring was performed at a central site using QualTek Molecular Laboratories, Pennsylvania. .. In addition, formalin-fixed paraffin-embedded samples and cryosections were co-stained for NKp46 (#MAB1850, R&D Systems, MN, USA) and granzyme B (#AF1865, R&D Systems) (full methods in and the supplementary CTAT table). .. Comprehensive image analysis was performed using HALO software v.3.4 (Indica Labs, NM, USA).



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    Effector functions of MSLN-specific T cells. (A) representative donor data (left panel) and summary results (median ± IQR, n=21; right panel) showing CD8 + /IFNγ + and CD4 + /IFNγ + T cells, as assessed by ICS. (B) representative donor data (left panel) and summary results (median ± IQR; n=21; right panel) showing dual (IFNγ/TNFα)-producing CD4 + and CD8 + MSLN-specific T cells, as assessed by ICS. Box plots show the median and 25th and 75th data quartiles. (C) polyfunctionality of MSLN-specific T cells as measured by <t>FluoroSpot</t> <t>(IFNγ/Granzyme</t> <t>B/TNFα)</t> (n=6). Top panel shows the total frequency (SFC/2x105 cells) of specific T cells for each of the individual effector molecules, while the bottom panel shows the proportion of cells that are single, dual or triple analyte-producing. All data represent biological replicates (i.e. independent donors). TNFα, tumor necrosis factor alpha; ICS, intracellular cytokine staining; GrB, Granzyme B.
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    Effector functions of MSLN-specific T cells. (A) representative donor data (left panel) and summary results (median ± IQR, n=21; right panel) showing CD8 + /IFNγ + and CD4 + /IFNγ + T cells, as assessed by ICS. (B) representative donor data (left panel) and summary results (median ± IQR; n=21; right panel) showing dual (IFNγ/TNFα)-producing CD4 + and CD8 + MSLN-specific T cells, as assessed by ICS. Box plots show the median and 25th and 75th data quartiles. (C) polyfunctionality of MSLN-specific T cells as measured by <t>FluoroSpot</t> <t>(IFNγ/Granzyme</t> <t>B/TNFα)</t> (n=6). Top panel shows the total frequency (SFC/2x105 cells) of specific T cells for each of the individual effector molecules, while the bottom panel shows the proportion of cells that are single, dual or triple analyte-producing. All data represent biological replicates (i.e. independent donors). TNFα, tumor necrosis factor alpha; ICS, intracellular cytokine staining; GrB, Granzyme B.
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    (A) FACS analysis of antigen specificity of FluTC generated from HLA-A2 + healthy donors by repetitive expansions. All samples were gated on lymphocytes, single cells, and live cells. CD8 and Flu-Pentamer stainings were performed on days 7 and 14 of antigen-specific expansion (ASE) and day 14 of rapid expansion protocol (REP). During ASE, some CD8 + T cells were expanded in the presence of unpulsed feeder cells (w/o flu-peptide) as a negative control. (B) <t>ELISA</t> to determine cytokine secretion by FluTC. MO3.13-A2-Luc cells were pulsed with diluting concentrations of flu peptide and co-cultured with FluTC. After 20 h of co-culture, the supernatant of co-culture was analyzed by ELISA to detect IFNγ (left) and <t>Granzyme</t> <t>B</t> (right) secretion by FluTC.
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    Image Search Results


    Effector functions of MSLN-specific T cells. (A) representative donor data (left panel) and summary results (median ± IQR, n=21; right panel) showing CD8 + /IFNγ + and CD4 + /IFNγ + T cells, as assessed by ICS. (B) representative donor data (left panel) and summary results (median ± IQR; n=21; right panel) showing dual (IFNγ/TNFα)-producing CD4 + and CD8 + MSLN-specific T cells, as assessed by ICS. Box plots show the median and 25th and 75th data quartiles. (C) polyfunctionality of MSLN-specific T cells as measured by FluoroSpot (IFNγ/Granzyme B/TNFα) (n=6). Top panel shows the total frequency (SFC/2x105 cells) of specific T cells for each of the individual effector molecules, while the bottom panel shows the proportion of cells that are single, dual or triple analyte-producing. All data represent biological replicates (i.e. independent donors). TNFα, tumor necrosis factor alpha; ICS, intracellular cytokine staining; GrB, Granzyme B.

    Journal: Frontiers in Immunology

    Article Title: Evaluating mesothelin as an immunotherapeutic target for endogenous T cells

    doi: 10.3389/fimmu.2026.1853409

    Figure Lengend Snippet: Effector functions of MSLN-specific T cells. (A) representative donor data (left panel) and summary results (median ± IQR, n=21; right panel) showing CD8 + /IFNγ + and CD4 + /IFNγ + T cells, as assessed by ICS. (B) representative donor data (left panel) and summary results (median ± IQR; n=21; right panel) showing dual (IFNγ/TNFα)-producing CD4 + and CD8 + MSLN-specific T cells, as assessed by ICS. Box plots show the median and 25th and 75th data quartiles. (C) polyfunctionality of MSLN-specific T cells as measured by FluoroSpot (IFNγ/Granzyme B/TNFα) (n=6). Top panel shows the total frequency (SFC/2x105 cells) of specific T cells for each of the individual effector molecules, while the bottom panel shows the proportion of cells that are single, dual or triple analyte-producing. All data represent biological replicates (i.e. independent donors). TNFα, tumor necrosis factor alpha; ICS, intracellular cytokine staining; GrB, Granzyme B.

    Article Snippet: For the quantitation of polyfunctional cells simultaneously secreting IFNγ, GrB and/or TNFα a commercial FluoroSpot assay was used (Human IFNγ/Granzyme B/TNFα FluoroSpot Plus, Mabtech, Inc.).

    Techniques: Staining

    (A) FACS analysis of antigen specificity of FluTC generated from HLA-A2 + healthy donors by repetitive expansions. All samples were gated on lymphocytes, single cells, and live cells. CD8 and Flu-Pentamer stainings were performed on days 7 and 14 of antigen-specific expansion (ASE) and day 14 of rapid expansion protocol (REP). During ASE, some CD8 + T cells were expanded in the presence of unpulsed feeder cells (w/o flu-peptide) as a negative control. (B) ELISA to determine cytokine secretion by FluTC. MO3.13-A2-Luc cells were pulsed with diluting concentrations of flu peptide and co-cultured with FluTC. After 20 h of co-culture, the supernatant of co-culture was analyzed by ELISA to detect IFNγ (left) and Granzyme B (right) secretion by FluTC.

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A) FACS analysis of antigen specificity of FluTC generated from HLA-A2 + healthy donors by repetitive expansions. All samples were gated on lymphocytes, single cells, and live cells. CD8 and Flu-Pentamer stainings were performed on days 7 and 14 of antigen-specific expansion (ASE) and day 14 of rapid expansion protocol (REP). During ASE, some CD8 + T cells were expanded in the presence of unpulsed feeder cells (w/o flu-peptide) as a negative control. (B) ELISA to determine cytokine secretion by FluTC. MO3.13-A2-Luc cells were pulsed with diluting concentrations of flu peptide and co-cultured with FluTC. After 20 h of co-culture, the supernatant of co-culture was analyzed by ELISA to detect IFNγ (left) and Granzyme B (right) secretion by FluTC.

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Generated, Negative Control, Enzyme-linked Immunosorbent Assay, Cell Culture, Co-Culture Assay